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placenta tissue lysates  (Merck & Co)

 
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    Merck & Co placenta tissue lysates
    Placenta Tissue Lysates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placenta+tissue+lysates/pm41772303-78-5-13?v=Merck+%26+Co
    Average 86 stars, based on 1 article reviews
    placenta tissue lysates - by Bioz Stars, 2026-08
    86/100 stars

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    All electropherograms report lysate concentrations of 0.5 and 1.0 mg/mL with 1:10 and 1:50 antibody concentrations. These detection antibodies were not close to their target protein’s predicted molecular weight and were, thus, not considered to be specifically binding target proteins of interest: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 ( D ) HSD17ß7 and ( E ) SULTe1.

    Journal: Methods and Protocols

    Article Title: Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue

    doi: 10.3390/mps5020034

    Figure Lengend Snippet: All electropherograms report lysate concentrations of 0.5 and 1.0 mg/mL with 1:10 and 1:50 antibody concentrations. These detection antibodies were not close to their target protein’s predicted molecular weight and were, thus, not considered to be specifically binding target proteins of interest: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 ( D ) HSD17ß7 and ( E ) SULTe1.

    Article Snippet: Control lysates tested include those for: HSD17ß1 (human placental whole cell lysate; NB820-59248, Novus Biologicals), HSD17ß2 (human embryonic kidney cells; NBL1-11726, Novus Biologicals), HSD17ß4 (human embryonic kidney cells; NBL1-11728, Novus Biologicals), HSD17ß7 (human embryonic kidney cells; NBP2-07042, Novus Biologicals) and SULTe1 (human embryonic kidney cells; ab94289, Abcam).

    Techniques: Molecular Weight, Binding Assay

    All electropherograms report results from over-expresser lysates at concentrations of 0.5 and 0.8 mg/mL ( A – D ) except for SULTe1 which was tested at: 1/50, 1/100, 1/200, 1/400, 1/800. Empty vector negative controls are also displayed on each electropherogram. Lysates were paired with antibody concentrations of 1:10 and 1:50. Data are listed as follows: ( A ) HSD17ß1, ( B ) HSD17ß2, ( C ) HSD17ß4, ( D ) HSD17ß7, and ( E ) SULTe1.

    Journal: Methods and Protocols

    Article Title: Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue

    doi: 10.3390/mps5020034

    Figure Lengend Snippet: All electropherograms report results from over-expresser lysates at concentrations of 0.5 and 0.8 mg/mL ( A – D ) except for SULTe1 which was tested at: 1/50, 1/100, 1/200, 1/400, 1/800. Empty vector negative controls are also displayed on each electropherogram. Lysates were paired with antibody concentrations of 1:10 and 1:50. Data are listed as follows: ( A ) HSD17ß1, ( B ) HSD17ß2, ( C ) HSD17ß4, ( D ) HSD17ß7, and ( E ) SULTe1.

    Article Snippet: Control lysates tested include those for: HSD17ß1 (human placental whole cell lysate; NB820-59248, Novus Biologicals), HSD17ß2 (human embryonic kidney cells; NBL1-11726, Novus Biologicals), HSD17ß4 (human embryonic kidney cells; NBL1-11728, Novus Biologicals), HSD17ß7 (human embryonic kidney cells; NBP2-07042, Novus Biologicals) and SULTe1 (human embryonic kidney cells; ab94289, Abcam).

    Techniques: Plasmid Preparation

    All electropherograms report data for high fat (75 SAT lysate:25 over-expresser lysate) and low fat (25 SAT lysate:75 over-expresser lysate) mixtures with 1:10 antibody concentrations in duplicate: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 and ( D ) SULTe1.

    Journal: Methods and Protocols

    Article Title: Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue

    doi: 10.3390/mps5020034

    Figure Lengend Snippet: All electropherograms report data for high fat (75 SAT lysate:25 over-expresser lysate) and low fat (25 SAT lysate:75 over-expresser lysate) mixtures with 1:10 antibody concentrations in duplicate: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 and ( D ) SULTe1.

    Article Snippet: Control lysates tested include those for: HSD17ß1 (human placental whole cell lysate; NB820-59248, Novus Biologicals), HSD17ß2 (human embryonic kidney cells; NBL1-11726, Novus Biologicals), HSD17ß4 (human embryonic kidney cells; NBL1-11728, Novus Biologicals), HSD17ß7 (human embryonic kidney cells; NBP2-07042, Novus Biologicals) and SULTe1 (human embryonic kidney cells; ab94289, Abcam).

    Techniques:

    Summary of expected and observed molecular weights for all 11 antibodies tested in each experiment.

    Journal: Methods and Protocols

    Article Title: Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue

    doi: 10.3390/mps5020034

    Figure Lengend Snippet: Summary of expected and observed molecular weights for all 11 antibodies tested in each experiment.

    Article Snippet: Control lysates tested include those for: HSD17ß1 (human placental whole cell lysate; NB820-59248, Novus Biologicals), HSD17ß2 (human embryonic kidney cells; NBL1-11726, Novus Biologicals), HSD17ß4 (human embryonic kidney cells; NBL1-11728, Novus Biologicals), HSD17ß7 (human embryonic kidney cells; NBP2-07042, Novus Biologicals) and SULTe1 (human embryonic kidney cells; ab94289, Abcam).

    Techniques: Extraction, Positive Control